Dr. Tsair-Wang Chung

Abstract: The antioxidant properties of the extract of saponin from Sapindus mukorossi (soapberry) was analyzed and optimized using Response Surface Methodology (RSM). In this study, the antioxidant properties were evaluated using different antioxidant tests. There are 1,1-di-phenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, reducing power, and ferrous ion chelating. The best DPPH free radical scavenging ability was obtained in 91.56 % (X1 = 1 hour, X2 = 1:15 (w/v), X3 = 0.55 %). The effects of solid-liquid ratio (X2) and enzyme concentration (X3) on the response of percentage of free radical scavenging (Y1) was significant. The best reducing power was obtained in 0.473 Abs (X1 = 3 hour, X2 = 1:10 (w/v), X3 = 0.1 %). The effects of solid-liquid ratio (X2) on the response of amount of reducing power (Y2) was significant. The best ferrous ion chelating ability was obtained in 49.63 % (X1 = 1 hour, X2 = 1:10 (w/v), X3 = 1 %). The effects of time (X1) and solid-liquid ratio (X2) on the response of percentage of ferrous ion chelating ability (Y3) was significant. The extract of saponin from Sapindus mukorossi shows excellent in antioxidant capacity based on reducing power and scavenging free radicals activity, but it is relatively weak in ferrous ion chelating.